PCR versus Stuhlkultur

Primer design versus PCR bias in methylation independent PCR amplifications.

Many protocols in methylation studies utilize one primer set to generate a PCR product from bisulfite modified template regardless of its methylation status (methylation independent amplification MIP). However, proportional amplification of methylated and unmethylated alleles is hard to achieve due to PCR bias favoring amplification of unmethylated relatively GC poor sequence. Two primer design...

Conventional multiplex polymerase chain reaction ( PCR ) versus real - time PCR for species - specific meat

Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...

Quantitative or semi-quantitative PCR: reality versus myth.

Johannes Bos University of Utrecht David Botstein Stanford University School of Medicine Jeff Chamberlain University of Michigan Medical School Henry Erlich Roche Molecular Systems Kenshi Hayashi National Cancer Research Institute, Tokyo Bernhard Horsthemke University of Essen Pieter de Jong Lawrence Livermore National Laboratory David Kemp Menzies School of Health Research Kenneth Kidd Yale Un...

Enterovirus RNA in Cerebrospinal Fluid Liquid-Phase Hybridization for Detection of Evaluation of Real-Time PCR versus PCR with

Evaluation of real-time PCR versus PCR with liquid-phase hybridization for detection of enterovirus RNA in cerebrospinal fluid.

A LightCycler and two TaqMan real-time PCR assays were evaluated against an older PCR with liquid-phase hybridization method for the detection of enterovirus RNA in 74 patient samples. The two-step LightCycler and the two-step TaqMan formats correlated well with each other (r(2) = 0.90) and were equally sensitive compared to the liquid-phase hybridization method, whereas the one-step recombinan...